HISTOLOGY OF SOMATIC EMBRYOS OF EURYCOMA LONGIFOLIA (SIMAROUBACEAE): RELEVANCE IN AGROBACTERIUM RHIZOGENES-MEDIATED TRANSFORMATION

摘要

Histological analysis conducted on somatic embryos of Eurycoma longifolia shows the developmental structures thatare remarkably similar to seeds found in the wild. The primary components of a growing somatic embryo are its shoot androot apical meristems indicated by dense layers of rapidly growing cells. The increased understanding of In vitro culturesystems and anatomical changes provide information into cellular processes that govern genetic transformation of E.longifolia with Agrobacterium rhizogenes. The presence of meristematic regions on cultured somatic embryos suggests thatthey are suitable for genetic transformation as genetic elements could be transported to these regions where growth anddifferentiation are centered. This allows the successful integration and expression of transferred DNA in the host organism,leading the way for an efficient A. rhizogenes-mediated transformation protocol.