SYNSEED PRODUCTION FOR STORAGE AND CONSERVATION OF OCHRADENUS BACCATUS DELILE

摘要

Ochradenus baccatus is a medicinal plant of high value, spread over sandy and stony places of Kingdom of SaudiArabia and most of the desert regions of Egypt. This species contains several flavanoids and specific constituents which areimportant as these have already been effectively used in lowering cholesterol in the blood of rats and high inhibitionpotential of the malarial parasite (Plasmodium falciparum). Synseeds were produced from stem segments and apical bud ofO. baccatus growing In vitro. Two sets of synseeds were produced, one non-dried and the other dried under runninglaminar air-flow for 30 min. Regeneration and regrowth were evaluated for 16 weeks storage under various temperatures (4,8 and 12oC). The maximum frequency of conversion into plantlets was achieved on the MS medium containing 1.0 μM BAin encapsulated nodal segments stored at 4oC. Rooting in these shoots was induced by the pulse treatment of 100 μM IBAfor 10 days, and the rooted shoots were transferred on the MS medium devoid of any PGR. Fair percent rooting occurredafter one week of transfer on the MS medium. Plantlets were successfully established. No phenotypic variations wereobserved between the synseed originated plants with mother plant. Genetic stability of synseed grown plants and motherplant was evaluated by inter-simple sequence repeat (ISSR) marker. The mother plant as well as regenerated plants fromsynseed resulted in a monomorphic banding pattern developed from ISSR markers confirming genetic stability among theclones. This protocol will help multiply and conserve the plant as well as for short-term storage of germplasm forcommercial use and exchange.