MOLECULAR ANALYSES OF THE GENERA EREMOPYRUM (LEDEB.) JAUB. & SPACH AND AGROPYRON GAERTNER (POACEAE) BY PCR METHODS

摘要

RAPD-PCR (Random Amplified Polymorphic DNA Polymerase Chain Reaction) and Post PCR (Polymerase ChainReaction) Melting Curve Analysis (MCA) have been used to investigate the pattern of genetic variation among some speciesin the genera Eremopyrum (Ledeb.) Jaub. & Spach and Agropyron Gaertner (Poaceae). Thirteen primers have been used inthe study based on the RAPD-PCR and MCA analyses. Each species produced a distinct pattern of DNA fragments whichhave been used as a measure of the degree of relationship between species by means of using the RAPD-PCR results withthree primers selected for identifying the genetic similarities. Polymorphic melting profiles have been obtained with PostPCR MCA method using three primers. Genetic similarities are calculated for all the species studied with RAPD-PCR andMCA methods, the dendrograms are obtained with the MVSP (Multi Variate Statistical Package) software using UPGMA(Unweighted Pair Group Method with Arithmetic Averages) and Jaccard’s Coefficient. Polymorphism between 18populations of Eremopyrum and 6 Agropyron populations and within the species are determined by using RAPD-PCR andPost PCR melting curve analysis (MCA) respectively.