ANTIBACTERIAL ACTIVITY OF FUMARIA INDICA (HAUSSKN.) PUGSLEY AGAINST SELECTED BACTERIAL STRAINS

摘要

Hydroponic setups are frequently developed and improved as they are convenient platforms for studying whole plantphysiology. Mostly, the available systems produce small amounts of plant material and are therefore, unsuitable for studiesrequiring large quantities of plant material like isolation of mitochondria. To address this issue, we have modified ahydroponic setup that can sustain hundreds of Arabidopsis and tobacco plants until adult plants are established. The setup isvery flexible and easy to construct. It is based on the use of recyclable and sterilizable plastic-net-pots and media containers,which are easily available from the local suppliers. The modified seed-pots and styrofoam sheets facilitate the transfer andharvesting of seedlings. We have used the Percoll based two-step density gradient centrifugation method for the isolation ofroot mitochondria from the hydroponically grown plants.