EXPLORING VALID REFERENCE GENES FOR QUANTITATIVE REAL-TIME RT-PCR STUDIES OF HYDROGEN PEROXIDE SIGNALING IN ARABIDOPSIS

摘要

ROP/RAC GTPases is a plant-specific subfamily of Rho GTPases that plays a versatile role in the regulation of plantgrowth, development, in hormone signal transduction and response to the environment. Prior to the present study, only oneRop gene in pepper has been described. However, with the recent release of the draft genome sequence of pepper allowes usto conduct a genome wide search to identify how many Rop family members existed in pepper genome. We carried outbioinformatics analysis to establish the conserved as well as divergent regions on the protein sequences, phylogeneticallyanalysis and the corresponding result shows that, CaROPs could be distributed into four groups as described in the literaturefor their homologs in Arabidopsis. To understand the function of nine Rop genes in pepper, we accordingly studied thetissue, fruit development and ripening expression patterns of CaRop genes by obtained RNA-seq data from public database.From our analysis, we realized that the expression of CaRop genes shows no total tissue or developmental specificexpression. Furthermore, gene expression profiles of CaRop in response to environment stresses and hormone treatment,such as inoculated with Ralstonia solanacearum, by heat stress as well as treated with four phytohormones respectively andevaluated with real time RT-PCR. The potential involvement of specific CaRop genes in growth, fruit development,ripening, environment stresses as well as hormone responses discussed and may lay the foundation for future functionalanalysis to unravel their biological roles.