A MULTI-GENE PHYLOGENY OF CERATOCYSTIS MANGINECANS INFECTING MANGO IN PAKISTAN

摘要

Mango trees (Mangifera indica L.) are affected by a serious wilt disease, recognized as mango sudden death first timereported in Muzafargargh Punjab, Pakistan in 1995. Its prevalent is in almost all mango growing areas with severity rangedfrom 2-5% in Punjab and 5-10% in Sindh. Survey and sampling was conducted during the year 2011-12, on mango orchidsin different distracts of Punjab and Sindh and no location was found free from this Disease. For molecular identification,DNA was successfully extracted and was then amplified by using ITS, BT, TEF (600-800)primers through PolymeraseChain Reaction (PCR) assay andnucleotide evidence of Pakistani isolates (45 for each gene) exhibiting the maximumgenetic homology with Ceratocystis manginecans (99-100%) followed by C. fimbriata (97%) and C. omanensis (80%)respectively. On the basics of morphological tools and comparison of nucleotide evidence of multi-genes, C. manginecans isdifferent from C. fimbriata and C. omanensis which infect mango in Pakistan. The availability of disease-free plantingmaterial and management in combination with fertilization and proper irrigation system would help in improving orchardmanagement system.