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Isolation of &i&Bacillus cereus&/i& in a Facility Preparing School Meals

机译:分离& i&芽孢杆菌& / i&在准备学校饭菜的设施中

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Food safety is a fundamental requirement in mass catering, as large numbers of meals are served each day to potentially vulnerable consumers, such as children. Food Business Operators implement plans for the microbiological monitoring of the meals prepared and served in the catering sector, and for the swab-sampling of surfaces. From January 2018 to June 2019, our laboratory analyzed both food and swab samples from four catering facilities. Considering the EFSA 2018 data, we specifically focused on samples analyzed for Bacillus cereus . Our data substantially showed episodic contamination due to a piece of equipment that is not usually subjected to microbiological control, thus suggesting that every aspect should be scrutinized in order to identify critical points. While Bacillus cereus is widespread in nature and common in soil, it is adapted for growth in the intestinal tracts of insects and mammals. It is often present in a variety of foods, and may cause an emetic or a diarrheal type of food-associated illness. B. cereus produces several toxins. Multiplex PCR enables seven toxin genes to be detected ( hblC , hblD , hblA , nheA , nheB , nheC and cytK ).
机译:食品安全是大规模餐饮的基本要求,每天供应大量的膳食,以潜在的脆弱的消费者,如儿童。食品业务运营商实施在餐饮部门准备和服务的膳食的微生物监测计划,以及表面的拭子采样。 2018年1月至2019年6月,我们的实验室分析了四种餐饮设施的食品和棉签样本。考虑到EFSA 2018数据,我们专注于分析为 芽孢杆菌的样品。我们的数据显着地显示出由于通常不受微生物控制的设备而导致的情节污染,从而表明应仔细审查每个方面以识别关键点。虽然 芽孢杆菌本质上是普遍的,但在土壤中普遍,它适用于昆虫和哺乳动物的肠道生长。它通常存在于各种食物中,并且可能导致刺激或腹泻类型的食物相关疾病。 B.豆类产生了几个毒素。多重PCR使检测七种毒素基因( HBLC, HBLD, HBLA, NHEA, NHEB, NHEC和 <我> cytk)。

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