IN VITRO REGENERATION OF VALERIAN (VALERIANA OFFICINALIS L.) INDUCED BY DIFFERENT CONCENTRATIONS OF NAA AND BAP

摘要

In this research, regeneration of Valeriana officinalis viashoot apex was studied. Shoot apex explants were cultured on MS medium supplementedwith different concentrations and combinations of NAA and BAP. The highest callusinduction percentage was obtained in the medium containing 0.5 mg/l BAP and 0.5mg/l NAA. Multiple shoot were induced from the shoot apex explant of V. officinalis byculturing them in MS medium supplemented with 0.5 mg/l of 6-benzylaminopurine(BAP). The explants also induced root formation in MS medium supplemented with1 mg/l of NAA. The highest fresh and dry weight of callus was observed when 0.5mg/l BAP was combined with 0.5 mg/l NAA. The addition of BAP up to 1 mg/l tothe medium containing different concentrations of NAA lead to the productionof callus which is compact rather than friable. This result suggests that thismethodology can be applied for rapid propagation of this species.