Improving the yield of Anoectochilus roxburghii by Bacillus licheniformis cultured in Agaricus bisporus industrial wastewater

摘要

BackgroundThere is a large amount of industrial wastewater produced by the mushroom industry during the canning processing each year, which could provide abundant carbon, nitrogen and inorganic salts for microbial growth. The aim of this study was to optimize the culture conditions forBacillus licheniformiscultured in theAgaricus bisporusindustrial wastewater to produce the agricultural microbial fertilizer.ResultsIn this work, the maximal biomass ofB. licheniformiscould be obtained under the following culture conditions: 33.7°C, pH?7.0, 221?rpm shaking speed, 0.5% wastewater, 2 (v:v, %) inoculum dose, loading liquid of 60?mL/250?mL and a culture time of 24?h, and the average experimental value obtained was 1.35?±?0.04?×?109?Obj/mL, which was within the 95% confidence interval of the predicted model (1.29–1.38?×?109?Obj/mL), and met the national microbial fertilizers' standard in China. Furthermore, the field experiment results showed that the fermentation broth ofB. licheniformiscould significantly improve the yield ofAnoectochilus roxburghii.ConclusionsAgaricus bisporusindustrial wastewater can be used to produce agricultural microbial fertilizer.