Substitution of a commercial diet with raw meat complemented with vegetable foods containing chickpeas or peas affects faecal microbiome in healthy dogs

摘要

The aim of the study was to investigate if the inclusion of chickpeas or peas in the diet canmodify faecal microbiome in dogs. Eight healthy adult Border collie, fed a commercial extrudeddiet as reference diet (RD), were divided in two groups of four individuals. At the beginning ofthe trial, one group received a diet based mainly of raw meat, rice and chickpeas (CP) and inthe other group this pulse was substituted with peas (PE). After 14 days, the dogs with CP dietshifted to the PE and those with PE shifted to the CP diet, for another 14 days. Faeces were collected at the beginning (T0), after 14 days (T14) and at the end of the study (T28). Faeces wereanalysed for 16S rRNA, short chain fatty acids (SCFA), lactate, pH and faecal score was also evaluated. The SCFA and lactate in the faeces were not affected by the inclusion of pulses, with theonly exception of isovalerate, which was higher in CP and PE diets in comparison with RD diet(p .05). The abundances of Erysipelotrichaceae incertae sedis, Eubacterium, Anaerobacter andSarcina significantly differed in CP and PE in comparison with RD. Moreover, the generaPrevotella, Lactobacillus, Alloprevotella, Suttarella varied significantly between CP and PE diets.The observed modifications of faecal microbioma were related not only to the change from RDto CP or PE, but also to the type of pulse, chickpeas or peas. However, long-term studies arerequired to investigate the implications that pulses can have for gut health.