Special Issue: Proceedings of the 17th ASPA Congress, Alghero, May 29-June 1, 2007

摘要

Abstract In this work we evaluated the combined use of AFLP markers and selective genotyping in a Grand Daughter experimental design (GDD), to identify chromosomal regions candidate to contain QTL for milk protein percentage (PP) in Italian Friesian cattle. We verified the approach by microsatellite analysis of 4 candidate chromosomal regions. Twenty-five AFLP primer combinations, assayed on the extreme minus and plus-variant half-sibs and on the grandsires of 2 GDD families, produced 180 polymorphic bands of paternal origin. Association analysis resulted in 16 AFLPs significantly associated to PP-EBVs (P≤0.05), that mapped on 8 chromosomes, in regions where PP-QTLs were identified in previous studies. Microsatellite analysis confirmed association for 2 regions of BTA10 and BTA28.