INDUCTION OF CALLOGENESIS AND SHOOTING IN ASTERACANTHA LONGIFOLIA(L.) NEES –A MEDICINAL HERB

摘要

This study is concerned to develop a rapid system for regenerating shoots and callus from mature shoot tip explants of Asteracantha longifolia (L.) Nees, a medicinally important annual herb (family acanthaceae). Effective shoot and callus regeneration was observed by using several concentrations of cytokinins and auxins with MS medium. Shoot tips responded better for callusing almost in all combinations such as BA + IAA (1.0+1.0, 1.5+1.0, 1.5+1.5, 2.0+1.5 mg/lit) and BA + NAA (1.0+1.0, 1.5+1.0, 1.5+1.5, 2.0+1.5 mg/lit). Shooting was observed only in shoot tip explants when media was supplemented with growth regulators in different combinations at different concentrations such as BA (0.5, 1.0, 1.5, 2.0 mg/lit) and BA+NAA (1.0+0.5, 1.5+0.5, 1.0+1.0, 1.5+1.0). However no shooting was observed in MS media by using nodal explants containing different concentrations of BA +NAA + IAA. This approach of plant tissue culture may proved to be a helpful method to develop a rapid system of regeneration for production of medicinally important products. Also the production of aseptic plants which may decline the over exploitation of plants growing under natural condition