Molecular characterization of mosquito in eastern Tripoli, Libya for species Culex pipiens, molestus and hybrids

摘要

Our study provides a molecular tool for identification of both Culex pipiens forms and their hybrids atSouq Al-jum’aa municipality east of Tripoli district / Libya. Notably, the two forms are morphologicallyindistinguishable but differ extensively in behavior and physiology and have different epidemiologicimportance. Mosquito samples were collected during 2016 from 10 sites within six months; molecularanalysis preceded using primers designed for the flanking region of microsatellite CQ11 to identify thetwo forms of Culex pipiens: and molestus compared to their hybrids. The genetic analysis of collectedsamples indicated that Culex pipiens species had the highest distribution among the other species atpercentage of 51% followed by Culex molestus at 35%, and 14% for the Hybrids. This molecularevidence was confirmative tool to establish the presence of both forms of Cx. pipiens and distinguishthem between the hybrids in Souq Al-jum’aa / Tripoli district.