Cloning and Activity Analysis of Grape VvCIPK10 Promoter

摘要

CBL/CIPK signaling system plays an important role in plant stress response proceeding. VvCIPK10 gene responds rapidly to low temperature and other stresses at transcriptional level. To find out how VvCIPK10 responds to the low temperature reaction, we cloned VvCIPK10 promoter gene in grapevine. Predictive analysis of cis-acting elements revealed that VvCIPK10 promoter sequence was rich in TATA-box and CAAT-box elements, as well as cis-acting elements related to light response, hormone response elements and low temperature response elements. After VvCIPK10 promoter fusion with GUS, the recombinant vector was transformed into tobacco leaves. GUS activity analysis showed that the promoter was active and its activity was induced by low temperature. The results of promoter truncation deletion test showed that 169 bp at 3' end of VvCIPK10 promoter was the key fragment in response to low temperature, and the low temperature response element played a decisive role in this fragment.