Simple Approach to Fabricate a Highly Sensitive H₂O₂ Biosensor by One-Step of Graphene Oxide and Horseradish Peroxidase Co-immobilized Glassy Carbon Electrode

摘要

We report a simple one-step adsorption method of horseradish peroxidase (HRP) and graphene oxide (GO) on a glassy carbon electrode (GCE) surface as a hydrogen peroxide (H2O2)-detecting biosensor. The surface of glassy carbon (GC) electrodes was evaluated by FT-IR spectroscopy, UV-vis spectrophotometry, atomic force microscopy (AFM), field emission scanning electron microscopy (FE-SEM) and electrochemical techniques. Under the optimum conditions, the biosensor could be successfully used for the amperometric determination of H2O2 in a linear concentration range of 2 ?M to 500 ?M with a detection limit of 1.6 ?M (S/N =3), and the response time was approximately 3 s. The fabricated biosensor can avoid interference from glucose, ascorbic acid, ethanol and uric acid.