Electrochemical Detection of VEGF165 Lung Cancer Marker Based on Au-Pd Alloy Assisted Aptasenor

摘要

As we know that bimetallic nanomaterials containing Pd exhibit distinct properties as well as remarkable catalytic capacity. In this paper, a facile one-pot hydrothermal co-reduction method was employed to synthesize the alloy and core-shell Au-Pd nanomaterials. Then, the Au-Pd alloy/ionic liquid (AP/IL) was utilized to immobilize the aptamer on a glassy carbon electrode as the proper nanocomposite platform, which was used to detect lung cancer factor (VEGF165) in the ultra-trace level. The differential pulse voltammogram was recorded under the signal-off mode, exhibiting a linearly relationship with the concentration of VEGF165 in the sample solution increasing from 1 to 150 pM, where the limit of the detection is 0.5 pM. However, a positive linear relationship was observed in the signal-on mode between the resistance of charge transfer and the concentration of VEGF165 ranging from 5 to 200 pM, where the limit of the detection was 0.78 pM. Moreover, the proposed sensor has been successfully used for determination of VEGF165 in human serum sample. The result indicate our proposed method could be potentially used for clinic diagnosis of lung cancer.