Effect of cu and co nanoparticles based complex on stratification of grape grafts

摘要

The aim of the given work was to investigate the influence of copper and cobalt nanoparticles on clonal micro reproduction of Johanniter grape technical variety received by method of isolated cells and tissues culture. Copper and cobalt nanoparticles were used in the form of colloidal solutions of high-viscosity (gels). Johanniter grape technical variety was used as an object of research. During experiments the optimum concentrations of copper (0.5, 1.0, 1.5 mg/l) and cobalt (0.5, 1.0, 1.5 mg/l) nanoparticles in colloidal solutions have been defined. These solutions were added in Murasige and Skoog nutrient medium. It is shown that 1 mg/l concentration of cobalt nanoparticle provides the highest coefficient of reproduction (1:17). The similar concentration of copper nanoparticles in the colloidal solution has not demonstrated a set positive dynamics.