Method of decellularized scaffold for tissue regeneration using tissue clearing

摘要

Super-resolution simultaneous visualization of diverse molecular species, such as proteins and mRNAs, is still challenging. In this work, we demonstrate the super-resolution simultaneous imaging of both proteins and mRNAs via expansion microscopy (ExM). To achieve it, we modify the protocol of expansion microscopy to bet- ter conserve the nanoscale details of both molecular species during the expansion process. We believe that this work will provide bet- ter understanding of molecular information of the brain and human body.