Anti-inflammatory Withanolides from Physalis minima

摘要

Five new withanolides (1–5 ) along with five known ones (6–10 ) were isolated from the whole plants of Physalis minima Linn. The chemical structures of the new compounds were identified as (20S ,22R ) 15a -acetoxy-5β,6β-epoxy-4β,14a ,28-trihydroxy-3β-methoxy-1-oxowitha-16,24-dienolide (1 ), (20S ,22R ) 15a -acetoxy-5β,6β-epoxy-3β,4β,14β,17β,20β-pentahydroxy-1-oxowitha-24-enolide (2 ), (20R ,22R ) 15α-acetoxy-4β,5α,6β,14α,20β-pentahydroxy-1-oxowitha-2,24-dienolide (3 ), (20R ,22R ) 15α-acetoxy-5α,6β,14α,20β-tetrahydroxy-1-oxowitha-2,24-dienolide (4 ), and (20S ,22R ) 5α,6β,14β-trihydroxy-1,15-dioxowitha-2,16,24-trienolide (5 ) on the basis of integration combining IR, UV, HR-ESI-MS, 1D-NMR, and 2D-NMR analyses. Biologically, compounds (1–10 ) were subjected to evaluate their anti-inflammatory activities via inhibiting nitric oxide production in lipopolysaccharide-stimulated murine RAW 264.7 cells in vitro . The activity screening indicated that all of the compounds showed a moderate inhibitory effect against nitric oxide production with IC_(50) values of 23.53–66.28 μM.