Study the Histological Changes in Intestine of Infected Rabbits with Entamoeba histolytica after Immunization with Klebsiella pneumonia K-Antigen

摘要

The purpose of this study is to examine the immunological influence of K-Antigen isolated from (Klebsiella pneumonia), in the intestine of rabbits that were inoculated in the laboratory with Entamoeba histolytica. In our study three groups of rabbits were used. The first group (Group I) has ten animals which were injected, subcutaneous and Intramuscular, with 1 ml of concentrations (0, 10, 20, 40.100) mg/ ml of K-antigen. After two weeks, Group I divided in two Group Ia and Group Ib, Group Ia(five rabbits) were dissected; the histological changes in the intestine were examined. The rest of the animals (Group Ib five rabbits) were feeding orally with 4×103?E.hisotlytica cysts to study the effect of immunization with K- antigen .Ten animals second group (Group II) was feeding orally by E.histolytica (positive control) to determine the infection dose and negative group (Group III) was feeding by (a physiological (saline) solution).The study showed inhibition of k-antigen for severs histological changes in the intestine following parasitic infection compared with positive control. We suggest the use k-antigen of K.pneumoniae as immunizer against infections with E.histolytica.