Microorganisms play pivotal roles within aquatic ecosystems, affecting their structure, functioning and services. However, little is known about the effects of water stratification and mixing on the aquatic microbial community dynamics in subtropical reservoirs. In this study, we explored vertical and seasonal patterns of microbial diversity in the Dongzhen Reservoir (southeast China). Quantitative PCR, quantitative RT-PCR, and 454 pyrosequencing were used for an in-depth characterization of the bacterial community across time (every three months for one year) and space (five different water depths). Our results indicated that thermal and oxygen stratification shaped the phylogenetic composition of microbial communities in the reservoir. There were significant differences in physical, chemical and microbiological parameters between epilimnion and hypolimnion ( P P
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机译：微生物在水生生态系统中起关键作用，影响其结构，功能和服务。然而，对于亚热带水库中水的分层和混合对水生微生物群落动态的影响知之甚少。在这项研究中，我们探索了东镇水库（中国东南部）的微生物多样性的垂直和季节性模式。定量PCR，定量RT-PCR和454焦磷酸测序用于跨时间（一年三个月一次）和空间（五个不同水深）的细菌群落的深入表征。我们的结果表明，热分层和氧气分层影响了储层中微生物群落的系统发育组成。 lim虫和hypo虫之间的物理，化学和微生物参数存在显着差异（P P
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