Hydropathy profile analyses of the amino acid sequence of the quorum-sensing hybrid histidine kinase LuxN of Vibrio harveyi predict a periplasmic location of the N terminus. To test this, two-hybrid proteins consisting of LuxN and an N-terminally fused maltose-binding protein with or without a leader sequence were analyzed with regard to the enzymatic activities of LuxN, protease accessibility, and complementation of an Escherichia coli malE mutant. The results strongly support a periplasmic location of the N terminus, implying that LuxN is anchored with nine transmembrane domains in the cytoplasmic membrane.
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机译：哈氏弧菌的群体感应杂合组氨酸激酶LuxN氨基酸序列的亲水性分析可以预测N末端的周质位置。为了测试这一点，分析了由LuxN和带有或不带有前导序列的N末端融合的麦芽糖结合蛋白组成的两个杂合蛋白，涉及LuxN的酶促活性，蛋白酶的可及性和大肠杆菌的互补性。 malE 突变体。结果强烈支持N末端的周质定位，这意味着LuxN在细胞质膜中锚定有9个跨膜结构域。
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