Pseudobactin Biogenesis in the Plant Growth-Promoting Rhizobacterium Pseudomonas Strain B10: Identification and Functional Analysis of the l-Ornithine N5 -Oxygenase (psbA) Gene

摘要

Pseudobactin_B10, the fluorescent siderophore produced by the rhizobacterium Pseudomonas strain B10, contains the hydroxamate ligandd-N5 -hydroxyornithine (d-N5 -OH-Orn). We cloned thel-Orn N5 -oxygenase (psbA) gene from a genomic library ofPseudomonas strain B10 and demonstrated that PsbA is involved in the conversion of l-Orn to itsN5 -OH derivative. PsbA shows significant similarity to microbial ω-amino acid hydroxylases containing flavin adenine dinucleotide and NADP cofactor-binding sites and the FATGY signature of the putative substrate recognition pocket. ThepsbA gene is monocistronic, and its transcription is negatively controlled by iron. A site-specific psbA mutant of Pseudomonas strain B10 was biochemically complemented with the precursor l-N5 -OH-Orn, suggesting that l-Orn is hydroxylated before conversion to the d isomer. The l-OrnN5 -hydroxylase-defective mutants ofPseudomonas strain B10 and Pseudomonas aeruginosa PAO1 were much less effective than the parental strains in suppressing the growth of the phytopathogen Erwinia carotovora in iron-poor medium. The extent of in vitro inhibition of E. carotovora was strictly iron dependent and directly correlated with the amount of released siderophores. These data strengthen the role of fluorescent siderophores in biocontrol of deleterious rhizomicroorganisms.