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Effect of Hook Subunit Concentration on Assembly and Control of Length of the Flagellar Hook ofSalmonella

机译:钩亚基浓度对沙门氏菌鞭毛钩组装和长度控制的影响

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The flagellar hook of Salmonella is a filamentous polymer made up of subunits of the protein FlgE. Hook assembly is terminated when the length reaches about 55 nm. After our recent study of the effect of cellular levels of the hook length control protein FliK, we have now analyzed the effect of cellular levels of FlgE itself. When FlgE was overproduced in a wild-type strain, afliC (flagellin) mutant, or a fliD(hook-associated protein 2 [HAP2], filament capping protein) mutant, the hooks remained at the wild-type length. In a fliK (hook length control protein) mutant, which produces long hooks (polyhooks), the overproduction of FlgE resulted in extraordinarily long hooks (superpolyhooks). In a flgK (HAP1, first hook-filament junction protein) mutant or a flgL (HAP3, second hook-filament junction protein) mutant, the overproduction of FlgE also resulted in longer than normal hooks. Thus, at elevated hook protein levels not only FliK but also FlgK and FlgL are necessary for the proper termination of hook elongation. When FlgE was severely underproduced, basal bodies without hooks were often observed. However, those hooks that were seen were of wild-type length, demonstrating that FlgE underproduction decreases the probability of the initiation of hook assembly but not the extent of hook elongation.
机译:沙门氏菌的鞭毛钩是一种丝状聚合物,由蛋白质FlgE的亚基组成。当长度达到约55 nm时,挂钩组件终止。在我们最近对钩长度控制蛋白FliK的细胞水平影响的研究之后,我们现在分析了FlgE自身的细胞水平的影响。当FlgE在野生型菌株中过量产生时,一个 fliC (鞭毛蛋白)突变体或一个 fliD (钩相关蛋白2 [HAP2],细丝覆盖蛋白)突变体,钩子保持在野生型长度。在产生长钩子(polyhooks)的 fliK (钩子长度控制蛋白)突变体中,FlgE的过度生产导致了异常长的钩子(超多钩子)。在 flgK (HAP1,第一钩-丝连接蛋白)突变体或 flgL (HAP3,第二钩-丝连接蛋白)突变体中,FlgE的过量生产也导致比普通钩子更长。因此,在升高的钩蛋白水平上,不仅FliK而且FlgK和FlgL对于钩终止的正确终止都是必需的。当FlgE严重不足时,经常观察到没有钩的基体。但是,看到的那些钩子具有野生型长度,表明FlgE生产不足会降低钩子组装开始的可能性,但不会降低钩子伸长的程度。

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