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首页> 外文期刊>Journal of bacteriology >Molecular cloning and regulation of expression of the genes for initiation factor 3 and two aminoacyl-tRNA synthetases.
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Molecular cloning and regulation of expression of the genes for initiation factor 3 and two aminoacyl-tRNA synthetases.

机译:起始因子3和两个氨酰基-tRNA合成酶的基因的分子克隆和表达的调控。

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A 22-kilobase fragment of the Escherichia coli chromosome which contains the genes for translation initiation factor 3, phenylalanyl-tRNA synthetase, and threonyl-tRNA synthetase was cloned into plasmid pACYC184. The hybrid plasmid (designated pID1) complements a temperature-sensitive pheS lesion in E. coli NP37. pID1-transformed NP37 overproduce initiation factor 3 and phenylalanyl-tRNA synthetase. Gene expression from pID1 was studied in vitro in a coupled transcription-translation system and in minicells. The results suggest that the genes for initiation factor 3 and phenylalanyl- and threonyl-tRNA synthetase are regulated by different mechanisms.
机译:将包含翻译起始因子3,苯丙氨酰-tRNA合成酶和苏氨酰-tRNA合成酶的基因的大肠杆菌染色体的22个碱基的片段克隆到质粒pACYC184中。杂种质粒(命名为pID1)可补充大肠杆菌NP37中对温度敏感的pheS病变。 pID1转化的NP37过度产生起始因子3和苯丙氨酰-tRNA合成酶。在耦合转录翻译系统和小细胞中体外研究了pID1的基因表达。结果表明,起始因子3和苯丙氨酰-和苏氨酰-tRNA合成酶的基因受不同机制调控。

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