Mutant strains of Neurospora crassa that lack trehalase and are unable to grow on trehalose were isolated, and the gene (tre) was positioned on the right arm of linkage group I. Maltase and β-galactosidase activities are almost identical in tre? strains, whereas that of invertase was reduced by more than half and those of acid phosphatase and amylase were somewhat increased. Heterocaryons between standard and trehalaseless strains yield less than one-tenth the activity of the former. In addition, strains with duplications heterozygous for trehalase produce less than 1% of the activity of the standard strain. An inhibitor of trehalase has been found in tre? strains; its sensitivity to heat and proteolysis, and its nondialyzability suggest that this substance is a protein. The mig gene, which determines the rate of migration of trehalase on acrylamide gels, has been shown to be less than 1 map unit away from the tre gene.
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机译：分离出缺乏海藻糖酶且不能在海藻糖上生长的 Neurospora crassa 突变株，并将基因（ tre ）定位在连锁群I的右臂。 β-半乳糖苷酶的活性在 tre β菌株中几乎相同，而转化酶的活性降低了一半以上，酸性磷酸酶和淀粉酶的活性有所提高。标准菌株和无海藻糖菌株之间的杂核子的活性不到前者的十分之一。此外，海藻糖酶杂合重复的菌株产生的活性不到标准菌株的1％。在 tre ？菌株中发现了海藻糖酶抑制剂。它对热和蛋白水解的敏感性以及不可透析性表明该物质是蛋白质。决定了海藻糖酶在丙烯酰胺凝胶上迁移速率的 mig 基因距离 tre 基因不到1个图单元。
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