Israeli, Ella (Rambam Hospital, Haifa, Israel). Mechanism of pock formation by Shope fibroma virus on monolayers of rabbit cells. J. Bacteriol. >92:727–732. 1966.—The mechanism of pock formation by the Shope fibroma virus (SFV) on rabbit cultures in vitro was studied with the use of p-fluorophenylalanine, 5-bromodeoxyuridine, and 5-iododeoxyuridine. The inhibitors were used to inhibit, and to initiate, virus replication at different times after infection. It was shown that pock formation required virus replication to a threshold value of 25 plaque-forming units per pock area, and that this amount of virus can be accumulated during a period about 3 days less than that required for pock formation. Inhibition of virus growth, and of cell multiplication, after this threshold has been reached, did not prevent pock development. A delay in the onset of virus growth required to reach the threshold virus content, caused an about equivalent delay in the time of pock formation. In the absence of inhibitors, pocks were not formed after infection of 84 rabbit embryo clones, or five mixtures of clones containing five to seven clones each. The results indicate that pock formation by SFV in vitro was the result of cell aggregation, and not of cell multiplication, in special types of cells.
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机译：以色列人Ella（以色列海法Rambam医院）。 Shope纤维瘤病毒在兔细胞单层上形成痘疱的机制。 J.细菌。 > 92： 727-732。 1966年。使用 p -氟代苯丙氨酸，5-溴脱氧尿苷和5-碘脱氧尿苷研究了Shope纤维瘤病毒（SFV）在体外兔培养物中形成痘疱的机制。抑制剂用于在感染后的不同时间抑制并引发病毒复制。结果表明，痘疱的形成需要病毒复制到每个痘疱区域25个噬菌斑形成单位的阈值，并且这种病毒的累积量可以比痘疱形成所需的时间短约3天。达到此阈值后，抑制病毒生长和细胞增殖并不能阻止麻痘的发展。达到阈值病毒含量所需的病毒生长延迟会导致麻子形成时间的延迟大致相同。在没有抑制剂的情况下，感染84个兔子胚胎克隆或5个克隆混合物（每个混合物包含5至7个克隆）后，不会形成po。结果表明，SFV体外形成的痘疱是特定类型细胞中细胞聚集的结果，而不是细胞增殖的结果。
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