首页> 外文期刊>Journal of cell biology >Isolation of a glycopeptide fraction from the surface of the sea urchin egg that inhibits sperm-egg binding and fertilization.
【24h】

Isolation of a glycopeptide fraction from the surface of the sea urchin egg that inhibits sperm-egg binding and fertilization.

机译:从海胆卵表面分离糖肽部分,抑制精子与卵子的结合和受精。

获取原文
       

摘要

The role of cell surface glycoproteins of the sea urchin egg in binding sperm has been examined by studying the biological activity of glycopeptides derived from these glycoproteins. Glycopeptides were produced from egg surface glycoproteins by Pronase digestion. After fractionation by gel filtration the glycopeptides were tested for their ability to inhibit the binding of sperm to eggs, presumably by competing with the egg surface glycoproteins for binding sites on the sperm. One glycopeptide fraction with an apparent molecular weight of approximately 6,000 was found to be a potent inhibitor of sperm-egg binding, as well as fertilization, even at nanomolar concentrations. This activity was heat stable and exerted its effect against the sperm and not the egg. Experiments with a radiolabeled form of the glycopeptide fraction directly demonstrated that at least one component of it bound to sperm. Specific binding of the radiolabeled glycopeptide occurred only to acrosome-reacted sperm. Because the isolated glycopeptide fraction has many of the characteristics that one would expect of a biologically active fragment of an egg surface receptor for sperm, these findings are consistent with the idea that one or more glycoconjugates on the surface of the egg are involved in sperm binding.
机译:海胆卵细胞表面糖蛋白在结合精子中的作用已通过研究衍生自这些糖蛋白的糖肽的生物学活性进行了研究。糖蛋白通过链酶消化从蛋表面糖蛋白产生。通过凝胶过滤分级分离后,测试糖肽抑制精子与卵结合的能力,大概是通过与卵表面糖蛋白竞争精子上的结合位点。发现一种表观分子量约为6,000的糖肽级分是即使在纳摩尔浓度下也能有效抑制精子与卵子结合以及受精的物质。这种活性是热稳定的,并且对精子而不是卵发挥作用。用放射性标记形式的糖肽部分进行的实验直接证明,它的至少一种成分与精子结合。放射性标记的糖肽的特异性结合仅发生在顶体反应的精子上。因为分离的糖肽级分具有人们期望的精子卵表面受体生物活性片段的许多特征,所以这些发现与卵子表面上的一种或多种糖结合物参与精子结合的想法是一致的。 。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号