Detection of equine arteritis virus in the semen of carrier stallions by using a sensitive nested PCR assay.

S A Gilbert; P J Timoney; W H McCollum; D Deregt

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Detection of equine arteritis virus in the semen of carrier stallions by using a sensitive nested PCR assay.

机译：通过使用敏感的巢式PCR检测法检测携带者种马精液中的马动脉炎病毒。

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A nested PCR, developed for the detection of equine arteritis virus (EAV) in semen, detected less than 2.5 PFU of EAV per ml of naturally infected seminal plasma. Based on results from testing 88 semen samples from 70 stallions, the sensitivity and specificity of the test were 100 and 97%, respectively.

机译：为检测精液中的马动脉炎病毒（EAV）而开发的巢式PCR，每毫升天然感染的精浆中的EAV含量不到2.5 PFU。根据对70个种马的88个精液样品进行测试的结果，测试的敏感性和特异性分别为100％和97％。

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《Journal of Clinical Microbiology》 |1997年第8期|共3页
作者
S A Gilbert; P J Timoney; W H McCollum; D Deregt;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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专利

1. RT-PCR AS EFFECTIVE DETECTION METHOD OF EQUINE ARTERITIS VIRUS IN THE SEMEN OF NATURALLY INFECTED STALLIONS [J] . MAGDALENA LARSKA, JERZY ROLA Bulletin of the Veterinary Institute in Pulawy . 2003,第2期

机译：RT-PCR作为自然感染种马精液中马鞭毛病毒的有效检测方法
2. Emergence of novel equine arteritis virus (EAV) variants during persistent infection in the stallion: Origin of the 2007 French EAV outbreak was linked to an EAV strain present in the semen of a persistently infected carrier stallion [J] . MiszczakF., LegrandL., BalasuriyaU.B.R., Virology . 2012,第2期

机译：种马持续感染过程中出现新的马动脉炎病毒（EAV）变异：2007年法国EAV爆发的原因与持续感染的携带者种马精液中存在的EAV株有关
3. Rapid and sensitive detection of equine arteritis virus in semen and tissue samples by reverse transcription-polymerase chain reaction, dot blot hybridisation and nested polymerase chain reaction. [J] . Starick E Acta Virologica: International Journal . 1998,第5期

机译：通过逆转录聚合酶链反应，斑点印迹杂交和巢式聚合酶链反应，快速，灵敏地检测精液和组织样品中的马动脉炎病毒。
4. Construction and characterization of a recombinant equine arteritis virus expressing the structural genes of porcine reproductive and respiratory syndrome virus [C] . Stephen J. Spatz, Carolyn A. Cook, John L. Alford Annual Meeting of the American Association of Swine Practitioners . 1999

机译：表达猪生殖和呼吸综合征病毒结构基因的重组标准动脉炎病毒的构建与鉴定
5. Evaluation of the safety of vaccinating mares against equine viral arteritis during mid or late gestation or during the immediate postpartum period and infection of embryos following insemination of donor mares with equine arteritis infective semen. [D] . Broaddus, Charles C. 2011

机译：评估母马在妊娠中期或晚期或产后立即接种疫苗预防马病毒性动脉炎的安全性，以及用马动脉炎感染精液授精供体母猪后对胚胎的感染。
6. Detection of equine arteritis virus in the semen of carrier stallions by using a sensitive nested PCR assay. [O] . S A Gilbert, P J Timoney, W H McCollum, 1997

机译：通过使用灵敏的巢式PCR检测法检测携带者种马精液中的马动脉炎病毒。
7. Detection of equine arteritis virus in the semen of carrier stallions by using a sensitive nested PCR assay [O] . S A Gilbert, P J Timoney, W H McCollum, 1997

机译：用敏感的巢式PCR测定检测载体血液的精液中的马动脉炎病毒
8. Development of the nested polymerase chain reaction (PCR) for detection of hepatitis C virus RNA in blood derivatives. Final report for the period 15 December 1994 - 15 December 1995 [R] . Pavelic, J. 1996

机译：用于检测血液衍生物中丙型肝炎病毒RNa的巢式聚合酶链反应（pCR）的开发。 1994年12月15日至1995年12月15日期间的最后报告

Detection of equine arteritis virus in the semen of carrier stallions by using a sensitive nested PCR assay.

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