Heterogeneity in imprinted methylation patterns of pluripotent embryonic germ cells derived from pre-migratory mouse germ cells

Tanya C. Shovlin; Gabriela Durcova-Hills; Azim Surani; Anne McLaren

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Heterogeneity in imprinted methylation patterns of pluripotent embryonic germ cells derived from pre-migratory mouse germ cells

机译：迁移前小鼠生殖细胞多能胚胎生殖细胞印迹甲基化模式的异质性

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Pluripotentstemcells,termedembryonicgerm(EG)cells,havebeengeneratedfrombothhumanandmouseprimordialgermcells(PGCs).Likeembryonicstem(ES)cells,EGcellshavethepotentialtodifferentiateintoallgermlayerderivativesandmayalsobeimportantforanyfutureclinicalapplications.ThedevelopmentofPGCseminvivo/emisaccompaniedbymajorepigeneticchangesincludingDNAdemethylationandimprinterasure.WehaveinvestigatedtheDNAmethylationpatternofseveralimprintedgenesandrepetitiveelementsinmouseEGcelllinesbeforeandafterdifferentiation.AnalysedcelllineswerederivedsoonafterPGCspecification,ldquo;earlyrdquo;,incomparisonwithEGcellsderivedafterPGCcolonisationofthegenitalridge,ldquo;laterdquo;andembryonicstem(ES)celllines,derivedfromtheinnercellmass(ICM).EarlyEGcelllinesshowedstrikinglyheterogeneousDNAmethylationpatterns,incontrasttotheuniformityofmethylationpatternseeninsomaticcells(control),lateEGcellandEScelllines.WealsoobservedthatallanalysedXXcelllinesexhibitedlessmethylationthanXY.WesuggestthatthisheterogeneitymayreflectthechangesinDNAmethylationtakingplaceinthegermcelllineagesoonafterspecification./p/div

机译：Pluripotentstemcells，termedembryonicgerm（EG）细胞，havebeengeneratedfrombothhumanandmouseprimordialgermcells（的PGC）.Likeembryonicstem（ES）细胞，EGcellshavethepotentialtodifferentiateintoallgermlayerderivativesandmayalsobeimportantforanyfutureclinicalapplications.ThedevelopmentofPGCs INVIVO isaccompaniedbymajorepigeneticchangesincludingDNAdemethylationandimprinterasure.WehaveinvestigatedtheDNAmethylationpatternofseveralimprintedgenesandrepetitiveelementsinmouseEGcelllinesbeforeandafterdifferentiation.AnalysedcelllineswerederivedsoonafterPGCspecification，ldquo; earlyrdquo;，incomparisonwithEGcellsderivedafterPGCcolonisationofthegenitalridge，ldquo; laterdquo; andembryonicstem（ES细胞系（ICM）。早期EG细胞系显示出异质的DNA甲基化模式，这与体细胞中的甲基化模式（对照），EG细胞和ES细胞系的均匀性相反。我们还观察到，所有分析的XY都表明了甲基化程度极低。规格确定后不久就可以改变DNA甲基化在生殖细胞系中的地位。 展开▼

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《Developmental biology》 |2008年第2期|共页

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Tanya C. Shovlin; Gabriela Durcova-Hills; Azim Surani; Anne McLaren;
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中图分类生物科学;

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1. Heterogeneity in imprinted methylation patterns of pluripotent embryonic germ cells derived from pre-migratory mouse germ cells. [J] . Shovlin TC, Durcova Hills G, Surani A Developmental biology . 2008,第2期

机译：多能性胚胎生殖细胞的印记甲基化模式中的异质性来自于迁移前的小鼠生殖细胞。

2. The combination of retinoic acid and estrogen can increase germ cells genes expression in mouse embryonic stem cells derived primordial germ cells [J] . Neda Eskandari, Meysam Hassani Moghaddam, Mohammad Ali Atlasi, Biologicals: Journal of the International Association of Biological Standardization . 2018,第期

机译：视黄酸和雌激素的组合可以增加小鼠胚胎干细胞中的胚芽细胞基因表达衍生的原始生殖细胞

3. Evaluation of Novel Mouse-Specific Germ Cell Gene Expression in Embryonic Stem Cell-Derived Germ Cell-Like Cells In Vitro with Retinoic Acid Treatment [J] . Tabari Maryam Gholamitabar, Jorsaraei Seyed Gholam Ali, Ghasemzadeh-Hasankolaei Mohammad, Cellular reprogramming . 2018,第4期

机译：用视黄酸处理在体外评价胚胎干细胞衍生胚细胞样细胞的胚胎干细胞衍生胚细胞样细胞的表达

4. Pluripotent stem cells developed into regenerated tooth by organ germ method in combination with tooth germ-derived epithelium [C] . Morita Ritsuko, Nakao Kazuhisa, Ogawa Miho, International Symposium on Micro-NanoMechatronics and Human Science . 2007

机译：通过器官胚芽方法将多能干细胞产生与牙齿胚芽衍生的上皮组合的变种牙齿

5. Primordial Germ Cell Differentiation in Vitro: A Model for Understanding Epigenetic Reprogramming and Genome-Wide DNA Demethylation in Mouse Primordial Germ Cells. [D] . Vincent, John James. 2013

机译：原始生殖细胞的体外分化：一种模型，用于了解小鼠原始生殖细胞中的表观遗传重编程和全基因组DNA去甲基化。

6. Imprinting Status of GαS NESP55 and XLαs in Cell Cultures Derived from Human Embryonic Germ Cells: GNAS Imprinting in Human Embryonic Germ Cells [O] . Janet L. Crane, Michael J. Shamblott, Joyce Axelman, 2009

机译：GαSNESP55和XLαs在人胚生殖细胞衍生的细胞培养物中的印迹状态：GNAS在人胚生殖细胞中的印迹

7. Heterogeneity in imprinted methylation patterns of pluripotent embryonic germ cells derived from pre-migratory mouse germ cells [O] . Shovlin Tanya C., Durcova-Hills Gabriela, Surani Azim, 2008

机译：迁移前小鼠生殖细胞的多能胚胎生殖细胞印迹甲基化模式的异质性

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3. 小鼠原始生殖细胞源胚胎干细胞的分离培养和建系/小鼠体细胞核移植技术的初步研究/抗生殖免疫抗体与不明原因反复自然流产关系的研究/蛋白激酶C在人绒毛膜癌JAR细胞中作用的研究 [J] . . 交通医学 . 2003,第005期

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6. 小鼠胚胎生殖嵴生殖细胞体外分化为卵母细胞及其调控机理的研究 [C] . 潘庆杰 ,李兰 ,董焕声 . 第六次全国动物生物技术学术研讨会 . 2011

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1. 鸟类多能胚胎生殖细胞系 [P] . 中国专利： CN100386429C . 2008.05.07

2. 鸟类多能胚胎生殖细胞系 [P] . 中国专利： CN1373798A . 2002-10-09

3. Human embryonic pluripotent germ cells [P] . 外国专利： US6090622A . 2000-07-18

机译：人胚胎多能生殖细胞

4. Pluripotent embryonic germ cell line of birds [P] . 外国专利： DE60037107T2 . 2008-09-25

机译：鸟类的多能胚胎生殖细胞系

5. AVIAN PLURIPOTENT EMBRYONIC GERM CELL LINE [P] . 外国专利： EP1155114B1 . 2007-11-14

机译：禽类胚胎胚芽细胞系

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Heterogeneity in imprinted methylation patterns of pluripotent embryonic germ cells derived from pre-migratory mouse germ cells

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