The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts

Bryan P. Haines; Rajeev Gupta; C. Michael Jones; Dennis Summerbell; Peter W.J. Rigby

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The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts

机译：NLRR基因家族和小鼠发育：改良的差异显示PCR将NLRR-1鉴定为早期体细胞性成肌细胞中表达的基因

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Duringvertebrateembryogenesis,thesomitesformbysegmentationofthetrunkmesoderm,lateraltotheneuraltube,inananteriortoposteriordirection.Analysisofdifferentialgeneexpressionduringsomitogenesishasbeenproblematicduetothelimitedamountoftissueavailablefromearlymouseembryos.Tocircumventtheseproblems,wedevelopedamodifieddifferentialdisplayPCRtechniquethatishighlysensitiveandyieldsproductsthatcanbeuseddirectlyasinsituhybridisationprobes.Usingthistechnique,weisolatedemNLRR-1/emasageneexpressedinthemyotomeofdevelopingsomitesbutnotinthepresomiticmesoderm.Detailedexpressionanalysisshowedthatthisgenewasexpressedintheskeletalmuscleprecursorsofthemyotome,branchialarchesandlimbsaswellasinthedevelopingnervoussystem.SomiticexpressionoccursintheearliestmyoblaststhatoriginatefromthedorsallipinapatternreminiscentofthemuscledeterminationgeneemMyf5/em,butnotattheventrallip,indicatingthatemNLRR-1/emisexpressedinasubsetofmyotomecells.TheemNLRR/emgenescompriseathree-genefamilyencodingglycosylatedtransmembraneproteinswithexternalleucine-richrepeats,afibronectindomain,animmunoglobulindomainandshortintracellulartailscapableofmediatingproteinndash;proteininteraction.AnalysisofemNLRR-3/emexpressionrevealedregulatedexpressionintheneuralsystemindevelopinggangliaandmotorneurons.emNLRR-2/emexpressionappearstobepredominatelyconfinedtotheadult.Theregulatedembryonicexpressionandcellularlocationoftheseproteinssuggestimportantrolesduringmousedevelopmentinthecontrolofcelladhesion,movementorsignalling./p/div

机译：Duringvertebrateembryogenesis，thesomitesformbysegmentationofthetrunkmesoderm，lateraltotheneuraltube，inananteriortoposteriordirection.Analysisofdifferentialgeneexpressionduringsomitogenesishasbeenproblematicduetothelimitedamountoftissueavailablefromearlymouseembryos.Tocircumventtheseproblems，wedevelopedamodifieddifferentialdisplayPCRtechniquethatishighlysensitiveandyieldsproductsthatcanbeuseddirectlyasinsituhybridisationprobes.Usingthistechnique，weisolated <位置> NLRR-1 的asageneexpressedinthemyotomeofdevelopingsomitesbutnotinthepresomiticmesoderm.Detailedexpressionanalysisshowedthatthisgenewasexpressedintheskeletalmuscleprecursorsofthemyotome，branchialarchesandlimbsaswellasinthedevelopingnervoussystem.Somiticexpressionoccursintheearliestmyoblaststhatoriginatefromthedorsallipinapatternreminiscentofthemuscledeterminationgene <位置> Myf5的的，butnotattheventrallip，indicatingthat <位置> NLRR -1 在成肌细胞亚群中表达。 NLRR 基因由编码糖基化的三基因家族组成ansmembraneproteinswithexternalleucine-richrepeats，afibronectindomain，animmunoglobulindomainandshortintracellulartailscapableofmediatingproteinndash ;. proteininteraction.Analysisof <位置> NLRR-3 的expressionrevealedregulatedexpressionintheneuralsystemindevelopinggangliaandmotorneurons <位置> NLRR-2 的expressionappearstobepredominatelyconfinedtotheadult.Theregulatedembryonicexpressionandcellularlocationoftheseproteinssuggestimportantrolesduringmousedevelopmentinthecontrolofcelladhesion，movementorsignalling 。 展开▼

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《Developmental biology》 |2005年第2期|共页

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Bryan P. Haines; Rajeev Gupta; C. Michael Jones; Dennis Summerbell; Peter W.J. Rigby;
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中图分类生物科学;

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1. The NLRB gene family and mouse development: Modified differential display PCR identifies NLRB-1 as a gene expressed in early somitic myoblasts [J] . Haines BP, Gupta R, Jones CM, Developmental biology . 2005,第2期

机译：NLRB基因家族和小鼠发育：改良的差异显示PCR将NLRB-1鉴定为早期体细胞性成肌细胞中表达的基因

2. Differential expression of COUP-TFI, CHL1, and two novel genes in developing neocortex identified by differential display PCR. [J] . Liu Q, Dwyer ND, OLeary DD The Journal of Neuroscience: The Official Journal of the Society for Neuroscience . 2000,第20期

机译：COUP-TFI，CHL1和两个新基因在差异显示PCR鉴定的新皮层发育中的差异表达。

3. Identification of age-dependently expressed genes in mouse liver by differential display-PCR analysis [J] . Tsubota E, Yasuda T, Iida R Comparative biochemistry and physiology, Part D. Genomics & proteomics . 2008,第1期

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4. VERIFICATION OF REAL-TIME PCR FOR DIFFERENTIAL EXPRESSION ANALYSIS OF THE SPS GENE FAMILY FROM ARABIDOPSIS THALIANA [C] . Luxraanan Selvanesan, Julian J. Eaton-Rye, Elspeth A. MacRae International Congress of Photosynthesis . 2005

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5. A complex program of dopamine-induced gene expression identified using differential-display PCR: Discovery of a novel inducible cyclin. [D] . Berke, Joshua Damien. 1998

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6. Differential Expression of COUP-TFI CHL1 and Two Novel Genes in Developing Neocortex Identified by Differential Display PCR [O] . Qing Liu, Noelle D. Dwyer, Dennis D. M. OLeary 2000

机译：COUP-TFICHL1和两个新基因在差异显示PCR鉴定的新皮层发育中的差异表达

7. The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts [O] . Haines Bryan P., Gupta Rajeev, Michael Jones C., 2005

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机译：核酸序列，氨基酸序列，复制性克隆载体，分离的宿主细胞表达载体，在宿主中测试作为骨调节治疗剂的物质的方法以鉴定参与骨调节的分子和候选蛋白以测试hbm活性，用于药物开发以治疗骨骼发育异常，治疗动物骨骼发育异常，改变宿主的骨骼发育，治疗骨质疏松症，评估对骨骼发育异常的遗传易感性诊断，鉴定遗传易患骨发育障碍，在骨组织中表达hbm蛋白，扩增核苷酸多态性，鉴定hbm基因的调节元件并调节骨密度，并且在一名患者中进行骨发育障碍的诊断测定，细菌人工染色体，分离的核酸片段，核酸和多肽。

2. Control primer annealing to improve the specificity of annealing in nucleic acid amplification, kit, methods for amplifying nucleic acid sequences of DNA and sequence of target nucleic acid or a mixture of nucleic acids, to detect the complementari Dade of DNA to mRNA differentially expressed in two or more samples of nucleic acid.To amplify a fragment of the cDNA target quickly, to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs to amplify rather than a sequence of nucleotide al VO at the same time, to produce a digital printing gDNA DNA, and RNA in a sample of mRNA.To identify segments of homology is conserved in a multigene family from a sample of mRNA, to identify a variation of nucleotide in a target nucleic acid, for mutagenesis in a target nucleic acid, and use of the initiator [P] . 外国专利： BRPI0214741B1 . 2015-05-12

机译：控制引物退火以改善核酸扩增中退火的特异性，试剂盒，扩增DNA核酸序列和靶核酸序列或核酸混合物的方法，检测DNA与mRNA互补表达的互补性（两种）或更多核酸样品。要快速扩增cDNA靶标的片段，以扩增与mRNA cDNA互补的长丝两倍全长的cDNA群体，并用与mRNA互补的5'双重修饰的长丝进行扩增而不是序列同时鉴定一个核苷酸的VO片段，以产生一个数字印刷的gDNA DNA和一个mRNA样品中的RNA。从一个mRNA样品中鉴定一个多基因家族中的同源片段是保守的，以鉴定一个核苷酸中一个核苷酸的变异。靶核酸，用于在靶核酸中诱变，以及引发剂的用途

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机译：控制引物退火，以提高核酸扩增试剂盒中退火的特异性。扩增DNA或核酸混合物的核酸序列的方法以及选择性扩增靶DNA或核酸混合物的核酸序列的方法用于检测DNA与两个或多个核酸样品中差异表达的mRNA的互补性的方法，扩增cDNA和DNA片段的方法可快速靶向。全长与mRNA cDNA互补的双细丝cDNA的cDNA群体，以及与mRNA互补的5'增强双细丝的cDNA，同时扩增而不是核苷酸靶序列的方法，产生数字印刷gDNA DNA的方法mRNA样品中的RNA和RNA.mRNA样品的多基因家族中用于鉴定同源片段的方法是保守的，这是一种在靶标中诱变的方法

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The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts

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