The timing of expression of four actin genes and an RNA polymerase III-transcribed repeated sequence is correct in hybrid embryos of the sea urchin species Strongylocentrotus purpuratus and Lytechinus pictus☆

摘要

TheaccumulationofthemRNAsfromfourStrongylocentrotuspurpuratusactingenes(thesinglemusclegeneM,andthreecytoskeletalgenesCyI,CyIIIa,andCyIIIb)andoftranscriptsfromanRNApolymeraseIII-transcribedrepeatedsequencefamily(SURF1)wasfollowedthroughouttheearlydevelopmentofhybridembryosofS.purpuratusandLytechinuspictus.EachoftheactinmRNAsappearedinhybridembryos,constructedineitherdirection(Spa?€?—Lpa??andLpa?€?—Spa??),atapproximatelythesametimethattheyappearinnormalS.purpuratusembryos.TranscriptsoftherepeatedsequencefamilySURF1alsoappearedatthecorrecttimeinthehybridembryos,butwerepresentatsubstantiallyreducedlevelswhencontributedbythepaternalgenome(Lpa?€?—Spa??).Theaccuratetemporalexpressionofthesegenesindicatesthatbothsetsofhybridembryoscontainfactorswhichregulatethetimingoftheirtranscription.