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首页> 外文期刊>Developmental biology >Vg1 has specific processing requirements that restrict its action to body axis patterning centers
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Vg1 has specific processing requirements that restrict its action to body axis patterning centers

机译:Vg1具有特定的处理要求,将其作用限制在身体轴构图中心

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Unlikemosttransforminggrowthfactor-β(TGF-β)superfamilymembers,Vg1hasbeenshownnottoproducegrossphenotypicalterationsinemXenopus/emembryoswhenoverexpressedbymRNAinjection.ExperimentswithartificialchimericconstructsandarecentlyidentifiedsecondalleleofVg1suggestthatthismaybeduetounusuallystringentrequirementsforproteolyticprocessing.WeprovidebiologicalandbiochemicalevidencethatcleavagebytwodistinctproteolyticenzymesisrequiredforeffectiveactivationofVg1.WedemonstrateatightlyrestrictedoverlapinexpressionpatternsofVg1withtheproteasesrequiredtoreleasethematurepeptide.Thedatapresentedmayaccountforthelong-standingobservationthatthevastmajorityofVg1protein,invivo,ispresentinitsunprocessedform.Takentogether,theseobservationsprovideaplausiblemechanismforlocalactionofVg1consistentwithrequirementsimposedbycurrentmodelsofpatternformationinthedevelopingbodyaxis./p/div
机译:Unlikemosttransforminggrowthfactor-β(TGF-β)superfamilymembers,Vg1hasbeenshownnottoproducegrossphenotypicalterationsin 爪蟾 embryoswhenoverexpressedbymRNAinjection.ExperimentswithartificialchimericconstructsandarecentlyidentifiedsecondalleleofVg1suggestthatthismaybeduetounusuallystringentrequirementsforproteolyticprocessing.WeprovidebiologicalandbiochemicalevidencethatcleavagebytwodistinctproteolyticenzymesisrequiredforeffectiveactivationofVg1.WedemonstrateatightlyrestrictedoverlapinexpressionpatternsofVg1withtheproteasesrequiredtoreleasethematurepeptide.Thedatapresentedmayaccountforthelong standingobservationthatthevastmajorityofVg1protein,体内,ispresentinitsunprocessedform.Takentogether,theseobservationsprovideaplausiblemechanismforlocalactionofVg1consistentwithrequirementsimposedbycurrentmodelsofpatternformationinthedevelopingbodyaxis。

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