首页> 外文期刊>The Journal of Experomental Medicine >Human B cell activation. Evidence for diverse signals provided by various monoclonal anti-IgM antibodies.
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Human B cell activation. Evidence for diverse signals provided by various monoclonal anti-IgM antibodies.

机译:人类B细胞活化。各种单克隆抗IgM抗体提供的各种信号的证据。

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Seven murine monoclonal antibodies (mAb) with different binding characteristics for human IgM varied markedly in their ability to induce proliferation of T cell-depleted human splenocytes. Two mAb (HB57 and 5D7) that bound to distinct epitopes on IgM were highly effective initiators of B cell proliferation at very low concentrations, in the presence of a T cell factor source. In the absence of T cell supernatant, both HB57 and 5D7 mAbs produced a markedly reduced degree of stimulation at all concentrations. Two additional anti-IgM mAb (VIIIE11 and Mu53) were distinctive in that, even at high concentrations, only limited proliferation was observed compared with the first group of mAb. This proliferation depended on the presence of T cell supernatant. Competitive-binding studies revealed that the epitope recognized by mAb Mu53 may be identical or very proximate to that recognized by HB57. Three other mAb (1G6, XG9, and P24) induced little or no proliferation. 1G6 bound to a unique epitope on the IgM molecule, whereas XG9 shared a determinant with VIIIE11 mAb. Regulatory influences of Fc receptor binding cannot account for all the diversity in proliferation observed with the individual anti-IgM mAb. Markedly augmented proliferation was obtained when B cells were cultured with certain combinations of anti-IgM mAb in the presence of exogenous T cell supernatant. The proliferation induced in the absence of T cell supernatant by high concentrations of mAb mixtures that included 1G6 approached that observed for the same mixtures in the presence of T cell supernatant. The data suggest that certain signals delivered through membrane IgM can bypass the need for T cell supernatant in the activation of human B lymphocytes.
机译:七种对人IgM具有不同结合特性的鼠单克隆抗体(mAb)在诱导T细胞耗竭的人脾细胞增殖的能力上有显着差异。在存在T细胞因子来源的情况下,以非常低的浓度结合到IgM上不同表位的两个mAb(HB57和5D7)是B细胞增殖的高效引发剂。在没有T细胞上清液的情况下,HB57和5D7 mAb在所有浓度下均产生明显降低的刺激程度。另外两种抗IgM mAb(VIIIE11和Mu53)的独特之处在于,即使在高浓度下,与第一组mAb相比也只能观察到有限的增殖。这种增殖取决于T细胞上清液的存在。竞争结合研究表明,mAb Mu53识别的表位可能与HB57识别的表位相同或非常接近。其他三种单克隆抗体(1G6,XG9和P24)几乎不诱导增殖。 1G6与IgM分子上的独特表位结合,而XG9与VIIIE11 mAb共享一个决定簇。 Fc受体结合的调节作用不能解释单个抗IgM mAb观察到的所有增殖多样性。当在外源性T细胞上清液存在下,将B细胞与抗IgM mAb的某些组合培养时,可获得明显增强的增殖。在不存在T细胞上清液的情况下,由高浓度的包含1G6的mAb混合物诱导的增殖接近在存在T细胞上清液的情况下对于相同混合物观察到的增殖。数据表明,通过膜IgM传递的某些信号可以绕过人类B淋巴细胞活化过程中对T细胞上清液的需要。

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