Evaluation of Cysteine Protease from Niger Seeds (Guizotiaabyssinica)

摘要

ABSTRACT Proteolytic enzymes play central role in the biochemical mechanism of germination and intricately involved in many aspects of plant physiology and development. To study the mechanism of protein mobilization, undertaken the task of purifying and characterizing proteases, which occur transiently in seeds of Niger. Cysteine protease (CPRNS) was purified to homogeneity with 118 fold by four step procedure comprising Crude extract, (NH4)2SO4 fractionation, DEAE-Cellulose and CM-sephacel chr.