Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast

摘要

TheconservedTACCproteinfamilylocalisestothecentrosome(thespindlepolebody,SPBinfungi)andmitoticspindles,therebyplayingacrucialroleinbipolarspindleassembly.However,itremainselusivehowTACCproteinsarerecruitedtothecentrosome/SPB.Here,usingfissionyeastAlp7/TACC,wehavedeterminedclusteredfiveaminoacidresidueswithintheTACCdomainrequiredforSPBlocalisation.Critically,thesesequencesareessentialforthefunctionsofAlp7,includingproperspindleformationandmitoticprogression.Moreover,wehaveidentifiedpericentrin‐likePcp1asaloadingfactortothemitoticSPB,althoughPcp1isnotasoleplatform...