Neohesperidin dihydrochalcone: Presentation of a small molecule activator of mammalian alpha‐amylase as an allosteric effector

摘要

Flavonoidsandtheirprecursortrans‐chalconehavebeenreportedasinhibitorsofmammalianalpha‐amylase.Withregardtothisbackground,neohesperidindihydrochalcone(NHDC)effectwasinvestigatedtowardporcinepancreaticalpha‐amylase(PPA),andfoundtobeanactivatoroftheenzyme.Themaximalactivation(uptothreefold)wasfoundtooccurat4.8mMofNHDC,whichcouldbeconsideredtohaveahighactivationprofile,withregardtothealphaandbetaparameters(alpha1beta).NHDCisanon‐essentialactivatoroftheenzymeandbasedontheresultsobtainedfrommodelingtools,itissuggestedtointeractwithPPAatahydrophilicsitelocatedattheN‐terminal,farfromtheactivesiteoftheenzyme...