Properties of the detergent solubilised cytochrome c oxidase (cytochrome cbb 3) purified from Pseudomonas stutzeri

摘要

>Cytochrome cbb ₃ is a cytochrome c-oxidising isoenzyme that belongs to the superfamily of respiratory haem/copper oxidases. We have developed a purification method yielding large amounts of pure cbb ₃ complex from the soil bacterium Pseudomonas stutzeri. This cytochrome cbb ₃ complex consists of three subunits (ccoNOP) in a 1:1:1 stoichiometry and contains two b-type and three c-type haems. The protein complex behaves as a monomer with an overall molecular weight of 114.0±8.9 kDa and a s 0 _20,w value of 8.9±0.3 S as determined by analytical ultracentrifugation. Crystals diffracting to 5.0 Å resolution have been grown by the vapour diffusion sitting drop method to an average size of 0.1×0.1×0.3 mm. This is the first crystallisation report of a (cbb ₃)-type oxidase.