Identification of α‐subunit Lys201 and β‐subunit Lys115 at the ATP‐binding sites inEscherichia coli F1‐ATPase

摘要

>Binding of about 1 mol of adenosine triphosphopyridoxal to Escherichia coli F₁-ATPase resulted in the nearly complete inactivation of the enzyme [(1987) J. Biol. Chem. 262, 7686–7692]. About two thirds of the label was bound to the α-subunit, and the rest to the β-subunit. The present study revealed that Lys201 in the α-subunit and Lys155 in the glycinerich region of the β-subunit are the major sites labeled with this reagent. Thus, these two residues might be located close to the γ-phosphate of the bound ATP.