amp;alpha;-SUBUNIT COMPOSING amp;beta;-HEXOSAMINIDASE A (Hex A), Hex A HAVING THE SAME amp;alpha;-SUBUNIT, GENE, VECTOR AND TRANSFORMED CELL ENCODING THE SAME amp;alpha;-SUBUNIT

摘要

PROBLEM TO BE SOLVED: To provide a recombinant Hex A excellent in at least one of excellent thermostability and transport efficiency to a lysosome.;SOLUTION: The Hex A has an α-subunit composed of an amino acid sequence in which substitution or deletion of amino acid residues is carried out in an amino acid sequence described in sequence number 1. For example, 6 kinds of V519A(8), N518Y/V519A(B6), P429Q/K432R/N518Y/V519A(B8), P429Q/K432R/N518Y/V519A/F521Y(B9), P209Q/N228S/P229L/V230S and deletion (A2) of the 231st threonine (T) and S51N/A53T(G4) are cited as the substitution or deletion.;COPYRIGHT: (C)2008,JPO&INPIT