Three‐dimensional structure of (1–36)bacterioopsin in methanol—chloroform mixture and SDS micelles determined by 2D 1H‐NMR spectroscopy

摘要

>Spatial structures of proteolytic segment A (sA) of bacterioopsin of H. halobium (residues 1–36) solubilized in a mixture of methanol—chloroform (1:1), 0.1 M LiClO₄ organic mixture, or in perdeuterated sodium dodecyl sulfate (SDS) micelles, were determined by 2D 1H-NMR techniques, 324 and 400 NOESY cross-peak volumes were measured in NOESY spectra of sA in organic mixture and SDS micelles, respectively. The sA spatial structures were determined by local structure analysis, distance geometry calculation with program DIANA and systematic search for energetically allowed side chain rotamers consistent with NOESY cross-peak volumes. The structures of sA are similar in both milieus and have the right-handed α-helical region from Pro? to Met32 with root mean square deviation (RMSD) of 0.25 Å between backbone heavy atoms and fit well with Pro? to Met32 α-helical region in electron cryo-microscopy model of bacteriorhodopsin. The N-terminal region Ala2-Gly4 of sA in organic mixture has a fixed structure of two consecutive γ-turns as 2 * 2_?-helix (RMSD of 0.25 Å) stabilized by the Thr? NH⋯O=C Gln3 and Ile4 NH⋯O=C Ala2 hydrogen bonds while this region in SDS micelles has disordered structure with RMSD of 1.44 Å for backbone heavy atoms. The C-terminal region Gly33-Asp? of sA is disordered in both milieus. Torsion angles χ1 of sA were unequivocally determined for 13 (SDS) and 11 (organic mixture) of α-helical residues and arc identical in both milieus.