Primary structure of the α‐subunit of vacuolar‐type Na+‐ATPase inEnterococcus hirae Amplification of a 1000‐bp fragment by polymerase chain reaction

摘要

>A 1000‐bp fragment ofEnterococcus hirae genomic DNA was amplified by the polymerase chain reaction method, using the oligonucleotide primers designed from amino acid sequences of both amino‐terminal and a tryptic fragment of the Na+‐ATPase α‐subunit in this organism. DNA sequencing of this product revealed that the amino acid sequence of Na+‐ATPase α‐subunit is highly homologous to the corresponding sequences of large (α) subunits of vacuolar (archaebacterial) type H+‐ATPases, supporting our proposal [Kakinuma, Y. and Igarashi, K. (1990) FEBS Lett. 271, 97–101] that the Na+‐ATPase of this organism belongs to the vacuolar‐type ATPase.