Modification of α‐amylase from Bacillus licheniformis by the polyaldehyde derived from β‐cyclodextrine and α‐amylase thermostability

摘要

>The cleavage of β-cyclodextrine by sodium periodate at the seven 2–3 diols of the glucose unit gives rise to the polyaldehyde 1, used to modify α-amylase. The reductive modification of α-amylase from Bacillus licheniformis reduced the number of reactive lysine groups from 8 to 3.5 per mol of enzyme with an activity loss of 25% and increased the half-life at 80°C from 4.7 to 7.0 minutes.