Purification and partial characterization of α‐pheromone‐binding protein from a mating‐type cells in the yeast Saccharomyces cerevisiae

摘要

>Two kinds of proteinacious substances which bind to α pheromone were isolated from heat-shock extract of a mating type cells of Saccharomyces cerevisiae. One has M _r ∼300 000 and the other M _r 58 000. We purified the smaller binding protein by successive application of DEAE and CM-cellulose chromatography, gel filtration, and isoelectrofocusing. The purified binding protein showed a single band by polyacrylamide gel electrophoresis. This binding protein was detected in a cells but not in α cells.