Last step in the cooperative pathway for galactolipid synthesis in spinach leaves: Formation of monogalactosyldiacylglycerol with C18 polyunsaturated acyl groups at both carbon atoms of the glycerol

摘要

>In this paper, the formation of monogalactosyldiacylglycerol (MGDG) with C18 polyunsaturated acyl groups at both positions of the glycerol from phosphatidylcholine (PC) of liposomes was demonstrated by a three-step procedure: spinach chloroplasts were first incubated with liposomes of PC-containing polyunsaturated fatty acids at both positions of the glycerol in the presence of phospholipid transfer protein. Chloroplasts were then incubated with phospholipase C in order to form 1,2-diacylglycerol (1,2-DAG) from PC integrated in the chloroplast envelope. Finally, washed chloroplasts were incubated with UDP-gal and MGDG was formed by galactosylation of 1,2-DAG.