Über einen energieunabhängigen austausch der alkali‐ionen Li+, Na+, K+, Rb+ und Cs+ GEGEN H+ in der membran intakter und desintegrierter rattenlebermitochondrien

摘要

class="custom"> class="bullet">1.1. The energy-independent exchange of alkali metal cations against protons was investigated in intact and disintegrated mitochondria. The exchange is not specific for different alkali metal ions. class="bullet">2.2. In intact mitochondria the apparent K_m for the energy-independent exchange range from 5 to 25 mM (Cs+, Rb+, K+ 5–10 mM; Na+ 15 mM; Li+ 20 mM). At all ions studied the maximum release of protons was 15–20 nval/mg protein. class="bullet">3.3. In disintegrated mitochondria there are no differences between the alkali metal ions (apparent K_m 30 mM; maximum H+-release 50 nval/mg protein). class="bullet">4.4. Directly accessible binding sites seem to exist in the outer membrane. Binding sites in the inner membrane are accessible only after disintegration, but not after addition of valinomycin + rotenone. id="after-abstract"> class="accordion"> class="accordion__title">href="http://onlinelibrary.wiley.com/doi/10.1016/0014-5793(72)80764-6/epdf" class="accordion__control"> class="icon icon__double-chevron"> Continue reading full article

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class="article-info__copyright">FEBS Letters 24 (1972) 1873-3468 © 2015 Federation of European Biochemical Societies