Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels

摘要

Poly A containing RNA isolated from embryonic chick calvaria waa transferred from 6% formaldehyde 0.75% agarose gels to diazobenzyloxymethyl paper and the paper then hybridized to either nick translated pro α1 collagen cDNA clones, pCgl or pCg54, or to the nick translated pro α2 collagen cDNA clone , pCg45. From the mobilities of the bands hybridizing most strongly to each, pro α2 collagen mRNA was shown to be slightly larger than pro ml mRNA; they are 5100 and 4900 nucleotides long respectively. pCg54 also hybridized weakly to two bands of lower mobility, corresponding to RNAs 6.4 and 5.6 kb long. Neither pCg54 nor pCg45 hybridized to type II procollagen mRNA in poly A containing RNA isolated from embryonic chick sterna.