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A modified procedure for the preparation of di-and triribonucleotides from pancreatic ribonuclease digest of RNA

机译:一种从胰腺核糖核酸酶消化的RNA制备二和三核糖核苷酸的改良程序

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摘要

A novel procedure for the separation of oligonucleotides from pancreatic RNase-digest of RNA is described. The method involves a group-separation of uracil-containing and of cytosine-containing nucleotides on Dowex 50W. The obtained groups are further separated on DEAE-Sephadex A-25 by a linear gradient of NH4 HCO3.
机译:描述了从胰腺RNA酶消化的RNA中分离寡核苷酸的新方法。该方法包括在Dowex 50W上对含尿嘧啶和含胞嘧啶的核苷酸进行组分离。所得的基团在DEAE-Sephadex A-25上通过NH 4 HCO 3 的线性梯度进一步分离。

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