Stress protein activation by the cyclopentenone prostaglandin 15-deoxy-|[Delta]|12,14-prostaglandin J2 in human mesangial cells

摘要

Stress protein activation by the cyclopentenone prostaglandin 15-deoxy-12,14-prostaglandin J2 in human mesangial cells.BackgroundThe cyclopentenone prostaglandin 15-deoxy-12,14-prostaglandin J2 (15dPGJ2) affects mesangial proliferation, survival and production of proinflammatory proteins. During a survey of the mesangial cell proteome after treatment with 15dPGJ2, heat shock protein 70 (HSP70) was found to be the most conspicuously up-regulated protein, suggesting that stress proteins are key mediators or modulators of the effects of 15dPGJ2. Because cyclopentenone prostaglandins are highly reactive toward intracellular thiols, the role of intracellular thiol modification in the stress response to 15dPGJ2 was examined.