A rapid, selective and sensitive UPLC-MS/MS assay was established to determine the plasma concentrations of four steroidal saponins. Sprague-Dawley rats were allocated to four groups which were orally administered Anemarrhena asphodeloides extracts (ASE), ASE combined with macromolecular fraction (ASE-MF), ASE combined with small molecule fraction (ASE-SF) and ASE combined with small molecule and macromolecular fraction (ASE-SF-MF) containing approximately the same dose of ASE. At different time points, the concentration of timosaponin BII, anemarsaponin BIII, timosaponin AIII and timosaponin E1 in rat plasma were determined and main pharmacokinetic parameters including Cmax, Tmax, T1/2, AUC were calculated using the DAS 3.2 software package. The statistical analysis was performed using the Student’s t-test with p 0.05 as the level of significance. MF had no effect on the pharmacokinetic behaviors and parameters of four steroidal saponins. It was found that Cmax and AUC of four steroidal saponins in group ASE-SF and ASE-SF-MF, were significantly increased compared with those in group ASE. These results indicate that SF in A. asphodeloides extracts could increase the absorption and improve the bioavailability of the steroidal saponins.
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机译:建立了快速,选择性和灵敏的UPLC-MS / MS测定法,以确定四种甾体皂苷的血浆浓度。将Sprague-Dawley大鼠分为四组,分别口服知母知母提取物(ASE),ASE结合大分子级分(ASE-MF),ASE结合小分子级分(ASE-SF)和ASE结合小分子和大分子馏分(ASE-SF-MF)含有大约相同剂量的ASE。在不同的时间点,测定大鼠血浆中的皂甙BII,脱敏皂甙BIII,皂甙AIII和皂甙E1的浓度,并确定主要药代动力学参数,包括C max sub>,T max sub>,T使用DAS 3.2软件包计算 1/2 sub>的AUC。使用学生t检验进行统计学分析,以p <0.05为显着性水平。 MF对四种类固醇皂苷的药代动力学行为和参数没有影响。结果发现,ASE-SF和ASE-SF-MF组的4种甾体皂苷的C max sub>和AUC明显高于ASE组。这些结果表明,A。asphodeloides提取物中的SF可以增加甾体皂苷的吸收并提高其生物利用度。
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